In vitro Cytotoxic potential of Clerodendrum colebrookianum Walp. against Dalton’s Lymphoma cells
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Abstract
Traditionally used medicinal plants are of interest among researchers due to their rich diversity of phytochemicals with varied pharmacological importance. In the present study, we aimed to investigate the phytochemical constituents, antioxidant potential, and in vitro cytotoxicity of Clerodendrum colebrookeanum against Dalton’s Lymphoma (DL) cells. The standard phytochemical tests revealed the presence of important phytoconstituents in the methanol leaf extract such as carbohydrates, glycosides, phenols, tannins, polyphenols, flavonoids, terpenoids, and saponins. The antioxidant activity determined using DPPH as a free radical showed potent antioxidant ability compared to standard ascorbic acid. Moreover, the in vitro antitumor potential was determined using cell viability assays trypan blue and MTT whose results exhibited the cytotoxic potential of the extract against DL cells. A significantly (p<0.0001) low IC50 value was found compared to the half-maximal reduction ability of the reference drug, cisplatin. However, the viability of normal cells (splenocytes) was higher, indicating the specificity of extract towards cancerous cells. Thus, the outcome of the present study will lead to further exploration of the anticancer potential of C. colebrookianum through in vivo studies and isolation of active principle(s)
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References
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